Direct degradation of cellulosic biomass to bio-hydrogen from a newly isolated strain Clostridium sartagoforme FZ11. (September 2015)

• Record Type: Journal Article
• Authors: Zhang, Jing-Nan; Li, Yan-Hong; Zheng, Hui-Qin; Fan, Yao-Ting; Hou, Hong-Wei
• Is Part Of: Bioresource technology. Volume 192(2015)
• Page Start: 60
• Page End: 67
• Publication Date: 2015-09
• Subjects: Clostridium sartagoforme FZ11 -- Isolation and identification -- Bio-degradation -- Cellulosic biomass -- Fermentative hydrogen production; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.05.034
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A strain was isolated from a reactor feeding compost acclimated by MCC and corn stalk. The new strain was identified as Clostridium sartagoforme FZ11 by 16S rDNA. Strain FZ11 could directly convert MCC and raw corn stalk to H2 without pretreatment. Max. H2 yields of MCC, CMC and corn stalk were 77.2, 64.6 and 87.2 ml/g at 35 °C. The report had special significance to directly convert cellulosic biomass to H2 . Abstract: A mesophilic hydrogen-producing strain, Clostridium sartagoforme FZ11, had been newly isolated from cow dung compost acclimated using microcrystalline cellulose (MCC) for at least 30 rounds in an anaerobic bioreactor, and identified by the 16S rDNA gene sequencing, which could directly utilized various carbon sources, especially cellulosic biomass, to produce hydrogen. The maximum hydrogen yields from MCC (10 g/l) and carboxymethyl cellulose (CMC, 10 g/l) were 77.2 and 64.6 ml/g, separately. Furthermore, some key parameters of affecting hydrogen production from raw corn stalk were also optimized. The maximal hydrogen yield and substrate degradation rate from raw corn stalk were 87.2 ml/g and 41.2% under the optimized conditions with substrate concentration of 15 g/l, phosphate buffer of 0.15 M, urea of 6 g/l and initial pH of 6.47 at 35 °C. The result showed that the strain FZ11 would be an ideal candidate to directly convert cellulosic biomass into bio-hydrogen without substrate pretreatment.