Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch. (January 2016)

• Record Type: Journal Article
• Authors: Piccoli, M.; Urbani, L.; Alvarez-Fallas, M.E.; Franzin, C.; Dedja, A.; Bertin, E.; Zuccolotto, G.; Rosato, A.; Pavan, P.; Elvassore, N.; De Coppi, P.; Pozzobon, M.
• Is Part Of: Biomaterials. Volume 74(2016)
• Page Start: 245
• Page End: 255
• Publication Date: 2016-01
• Subjects: Extracellular matrix -- Decellularization -- Skeletal muscle -- Regenerative medicine; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2015.10.005
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able to allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold can generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations and M2 polarized macrophages that together lead to a local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in a xenogeneic setting, but it remains unclear whether it can be therapeutic in a model of muscle diseases. We demonstrated here for the first time that orthotopic transplantation of a decellularized diaphragmatic muscle from wild animals promoted tissue functional recovery in an established atrophic mouse model. In particular, ECM supported a local immunoresponse activating a pro-regenerative environment and stimulating host muscle progenitor cell activation and migration. These results indicate that acellular scaffolds may represent a suitable regenerative medicine option for improving performance of diseased muscles.