Genomic characterization of Nocardia seriolae strains isolated from diseased fish. Issue 3 (16th August 2018)

• Record Type: Journal Article
• Authors: Han, Hyun‐Ja; Kwak, Min‐Jung; Ha, Sung‐min; Yang, Seung‐Jo; Kim, Jin Do; Cho, Kyoung‐hee; Kim, Tae‐Wook; Cho, Mi Young; Kim, Byung‐Yong; Jung, Sung‐Hee; Chun, Jongsik
• Is Part Of: MicrobiologyOpen. Volume 8:Issue 3(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-08-16
• Subjects: Anguilla japonica -- Channa argus -- comparative genomics -- fish pathogen -- Norcardia seriolae; Microbiology -- Periodicals; 579
• DOI: 10.1002/mbo3.656
• Languages: English
• ISSNs: 2045-8827

Abstract

Abstract: Members of the genus Nocardia are widespread in diverse environments; a wide range of Nocardia species are known to cause nocardiosis in several animals, including cat, dog, fish, and humans. Of the pathogenic Nocardia species, N. seriolae is known to cause disease in cultured fish, resulting in major economic loss. We isolated two N. seriolae strains, CK‐14008 and EM15050, from diseased fish and sequenced their genomes using the PacBio sequencing platform. To identify their genomic features, we compared their genomes with those of other Nocardia species. Phylogenetic analysis showed that N. seriolae shares a common ancestor with a putative human pathogenic Nocardia species. Moreover, N. seriolae strains were phylogenetically divided into four clusters according to host fish families. Through genome comparison, we observed that the putative pathogenic Nocardia strains had additional genes for iron acquisition. Dozens of antibiotic resistance genes were detected in the genomes of N. seriolae strains; most of the antibiotics were involved in the inhibition of the biosynthesis of proteins or cell walls. Our results demonstrated the virulence features and antibiotic resistance of fish pathogenic N. seriolae strains at the genomic level. These results may be useful to develop strategies for the prevention of fish nocardiosis. Abstract : The genomes of two N. seriolae strains from diseased fish were completely sequenced and their genomic features were identified using a comparative approach with those of other Nocardia species.