Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae). (11th November 2018)

• Record Type: Journal Article
• Authors: Li, Wenjun; Su, Zhihao; Li, Airong; Guan, Kaiyun; Feng, Ying
• Is Part Of: Grassland science. Volume 65:Number 2(2019)
• Page Start: 135
• Page End: 138
• Publication Date: 2018-11-11
• Subjects: invasive native species -- Pedicularis kansuensis -- polymorphic microsatellites; Grasslands -- Periodicals; Grasslands -- Management -- Periodicals; 578.74
• DOI: 10.1111/grs.12222
• Languages: English
• ISSNs: 1744-6961

Abstract

Abstract: To quantify the population‐level genetic characteristics of Pedicularis kansuensi s, polymorphic microsatellite loci (simple sequence repeats) were identified and characterized. Methods and Results: Using Illumina HiSeq paired‐end reads from genomic DNA, searches for repeat motifs identified 1594 sequences by four softwares simultaneously. We developed and characterized 18 polymorphic microsatellite primers for P. kansuensis using 45 individuals that were collected from three P. kansuensi s populations. The number of alleles varied from two to 12. The observed heterozygosity and expected heterozygosity per locus ranged from 0.182 to 0.933 and 0.227 to 0.904 in the pooled population, respectively. All of the primers that were developed were also successfully applied in Pedicularis cheilanthifolia and Pedicularis violascens . These markers should be useful in probing the genetic diversity, genetic structure of P. kansuensis, which could provide information about assessment and prediction of potential risks of this species in its distribution areas.