Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes. Issue 4 (21st January 2019)

• Record Type: Journal Article
• Authors: Szafraniec, Ewelina; Kus, Edyta; Wislocka, Adrianna; Kukla, Bozena; Sierka, Ewa; Untereiner, Valérie; Sockalingum, Ganesh D.; Chlopicki, Stefan; Baranska, Malgorzata
• Is Part Of: Journal of biophotonics. Volume 12:Issue 4(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-01-21
• Subjects: hepatocytes -- lipid droplets -- liver sinusoidal endothelial cell -- NAFLD -- Raman imaging; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201800290
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Liver sinusoidal endothelial cells (LSECs), a type of endothelial cells with unique morphology and function, play an important role in the liver hemostasis, and LSECs dysfunction is involved in the development of nonalcoholic fatty liver disease (NAFLD). Here, we employed Raman imaging and chemometric data analysis in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine LSECs, in comparison with hepatocytes, isolated from mice on high‐fat diet. On NAFLD development, LDs content in LSECs changed toward more unsaturated lipids, and this response was associated with an increased expression of stearylo‐CoA desaturase‐1. To the best of our knowledge, this is a first report characterizing LDs in LSECs, where their chemical composition is analyzed along the progression of NAFLD at the level of single LD using Raman imaging. Abstract : In presented work, the Raman imaging and chemometric data analysis was employed in order to characterize the presence of lipid droplets (LDs) and their lipid content in primary murine liver sinusoidal endothelial cells (LSECs), in comparison with hepatocytes, isolated from mice on high‐fat diet (HFD). In mice on HFD, LDs content in LSECs changed toward more unsaturated lipids, and this response was associated with an increased expression of stearylo‐CoA desaturase‐1 in LSECs.