An innovative silicon-chip for sensitive real time PCR improvement in pathogen detection. Issue 7 (21st February 2019)

• Record Type: Journal Article
• Authors: Battaglia, Simone; Petralia, Salvatore; Vicario, Nunzio; Cirillo, Daniela; Conoci, Sabrina
• Is Part Of: Analyst. Volume 144:Issue 7(2019)
• Page Start: 2353
• Page End: 2358
• Publication Date: 2019-02-21
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c9an00006b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : An innovative miniaturized silicon-chip was developed for highly sensitive detection of pathogen genomes of both viruses and bacteria through real time PCR (qRT-PCR). Abstract : An innovative miniaturized silicon-chip was developed for highly sensitive detection of pathogen genomes of both viruses and bacteria through real time PCR (qRT-PCR). The device was properly designed to enhance the optical signal and perform accurate thermal control. Results show an improvement of PCR amplification by one order of magnitude in sensitivity compared to the standard RT-PCR method. In particular for hepatitis B virus a decrease of the mean value of Ct of about 2.9 ± 0.9 compared to the standard system was observed. Similarly, for the bacteria Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter baumannii, a decrease of the mean values of Ct of 1.8 ± 0.5, 3.1 ± 0.5 and 3.9 ± 0.9, respectively, was observed.