A mitochondria-targeted red-emitting probe for imaging hydrogen sulfide in living cells and zebrafish. Issue 13 (12th March 2019)

• Record Type: Journal Article
• Authors: Ismail, Ismail; Wang, Dan; Wang, Dawei; Niu, Cuili; Huang, Haojie; Yi, Long; Xi, Zhen
• Is Part Of: Organic & biomolecular chemistry. Volume 17:Issue 13(2019)
• Page Start: 3389
• Page End: 3395
• Publication Date: 2019-03-12
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c8ob03219j
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A mitochondria-targeted red-emitting probe is designed and prepared for H2 S detection in living cells and zebrafish. Abstract : Hydrogen sulfide (H2 S) is an important endogenous signaling molecule with multiple biological functions. Based on the thiolysis of NBD (7-nitro-1, 2, 3-benzoxadiazole) amine, herein we rationally design and synthesize a new fluorescent probe1 for the detection of mitochondrial H2 S in living cells. Probe1 displays bright red-emitting fluorescence after H2 S activation ( ϕ, 0.77).1 shows higher selectivity and sensitivity for H2 S than the red-emitting probeRho-NBD ( ChemBioChem, 2016, 17, 962). Moreover, 1 is water-soluble, cell-membrane-permeable, of low cytotoxicity and mitochondria-targeting, and can be employed for monitoring mitochondrial H2 S in living cells.