Inertial focusing with sub-micron resolution for separation of bacteria. Issue 7 (1st March 2019)

• Record Type: Journal Article
• Authors: Cruz, Javier; Graells, Tiscar; Walldén, Mats; Hjort, Klas
• Is Part Of: Lab on a chip. Volume 19:Issue 7(2019)
• Page Start: 1257
• Page End: 1266
• Publication Date: 2019-03-01
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c9lc00080a
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Inertial focusing in curved channels is demonstrated for particles between 0.5 and 2.0 μm in diameter; a range of biological relevance since it comprises a multitude of bacteria and organelles of eukaryotic cells. Abstract : In this paper, we study inertial focusing in curved channels and demonstrate the alignment of particles with diameters between 0.5 and 2.0 μm, a range of biological relevance since it comprises a multitude of bacteria and organelles of eukaryotic cells. The devices offer very sensitive control over the equilibrium positions and allow two modes of operation. In the first, particles having a large variation in size are focused and concentrated together. In the second, the distribution spreads in a range of sizes achieving separation with sub-micron resolution. These systems were validated with three bacteria species ( Escherichia coli, Salmonella typhimurium and Klebsiella pneumoniae ) showing good alignment while maintaining the viability in all cases. The experiments also revealed that the particles follow a helicoidal trajectory to reach the equilibrium positions, similar to the fluid streamlines simulated in COMSOL, implying that these positions occupy different heights in the cross section. When the equilibrium positions move to the inner wall as the flow rate increases, they are at a similar distance from the centre than in straight channels (∼0.6 R ), but when the equilibrium positions move to the outer wall as the flow rate increases, they are closer to the centre and the particles pass close to the inner wall to elevate their position before reaching them. These observations were used along with COMSOL simulations to explain the mechanism behind the local force balance and the migration of particles, which we believe contributes to further understanding of the phenomenon. Hopefully, this will make designing more intuitive and reduce the high pressure demands, enabling manipulation of particles much smaller than a micrometer.