Biodegradation of poly(ε-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. (April 2019)

• Record Type: Journal Article
• Authors: Mandic, Mina; Spasic, Jelena; Ponjavic, Marijana; Nikolic, Marija S.; Cosovic, Vladan R.; O'Connor, Kevin E.; Nikodinovic-Runic, Jasmina; Djokic, Lidija; Jeremic, Sanja
• Is Part Of: Polymer degradation and stability. Volume 162(2019)
• Page Start: 160
• Page End: 168
• Publication Date: 2019-04
• Subjects: Biopolymers -- Enzymes -- Pseudomonas -- Streptomyces -- Biodegradation -- Compost; ATR-FTIR Attenuated total teflectance-fourier transform infrared spectroscopy -- PHA Polyhydroxyalkanoates -- scl-PHA Short-chain length polyhydroxyalkanoates -- mcl-PHA Medium-chain length polyhydroxyalkanoates -- lcl-PHA Long-chain length polyhydroxyalkanoates -- PHB polyhydroxybutyrate -- PCL Poly(ε-caprolactone); Polymers -- Deterioration -- Periodicals; Stabilizing agents -- Periodicals; Polymères -- Dégradation -- Périodiques; Stabilisants -- Périodiques; 668.9
• DOI: 10.1016/j.polymdegradstab.2019.02.012
• Languages: English
• ISSNs: 0141-3910

Abstract

Abstract: Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly( ε -caprolactone) (PCL) or polyhydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 μm) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 μm) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chlororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis B-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers. Graphical abstract: Image 1 Highlights: Pseudomonas and Streptomyces grown on waste cooking oil for biopolymers degradation. Enzymes from Pseudomonas chlororaphis B-561 hydrolyzed PCL better than mcl-PHA. Mcl-PHA degraded more efficiently in model compost with Streptomyces sp. BV315.