SCCmec typing of PVL-positive community-acquired Staphylococcus aureus (CA-MRSA) at a Japanese hospital. Issue 3 (March 2019)

• Record Type: Journal Article
• Authors: Funaki, Toshitaka; Yasuhara, Tsutomu; Kugawa, Satoshi; Yamazaki, Yohei; Sugano, Emi; Nagakura, Yoshimi; Yoshida, Katsuhiko; Fukuchi, Kunihiko
• Is Part Of: Heliyon. Volume 5:Issue 3(2019)
• Publication Date: 2019-03
• Subjects: Epidemiology -- Infectious disease -- Microbiology; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2019.e01415
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: The epidemiology of Panton-Valentine leukocidin (PVL)-positive MRSA in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was examined. Three hundred and forty-two CA-MRSA strains that were susceptible to imipenem and cefazolin were isolated from 1107 samples (intravenous catheter, blood, sputum, urine, skin, wound, and pharynx) from outpatients at Showa University Hospital in Japan between September 2009 and March 2017. The PVL gene was detected in 46 of 342 CA-MRSA strains, accounting for 13.5%. The type of SCC mec was determined by detection of each SCC mec -specific region, class complex, and ccr . SCC mec type IV comprised 33 strains, type V comprised 5 strains, type VII comprised 4 strains, and the unclassified type comprised 4 strains. Among the type IV strains, subtype IVa was dominant, comprising 23 of 33 strains, and the remaining 10 strains were of varying subtypes. The SCC mec type III-specific region, CZ049, was amplified in 2 type V strains, 4 type VII strains, and 4 unclassified strains. In 4 unclassified strains, CZ049 and ccr5 were detected, but neither the SCC mec -specific region nor class complex was detected. The PVL-positive rate was lower than that in Western countries. The SCC mec types of PVL-positive CA-MRSA strains were found to vary, indicating a diverse spreading route.