Improvement of protein binding capacity of acrylic-acid-grafted fibers by polymer root-to-brush shift. (May 2019)

• Record Type: Journal Article
• Authors: Matsuzaki, Yuka; Itabashi, Takeshi; Kawai-Noma, Shigeko; Umeno, Daisuke; Saito, Kyoichi
• Is Part Of: Radiation physics and chemistry. Volume 158(2019:May)
• Page Start: 131
• Page End: 136
• Publication Date: 2019-05
• Subjects: Radiation-induced graft polymerization -- Acrylic-acid-grafted fiber -- Protein-binding capacity -- Conditioning with alkaline solution -- Immersion in hot water; Radiation chemistry -- Periodicals; Radiometry -- Periodicals; Radiation -- Periodicals; Chimie sous rayonnement -- Périodiques; 539.2
• DOI: 10.1016/j.radphyschem.2018.12.022
• Languages: English
• ISSNs: 0969-806X

Abstract

Abstract: A weak acid cation exchange fiber used for large-scale antibody purification was prepared by radiation-induced graft polymerization of acrylic acid (AA) onto a commercially available nylon-6 fiber. The AA-grafted fiber was post-treated by conditioning in NaOH aqueous solution at 298 K followed by immersion in water at 353 K to increase the protein binding capacity of the cation exchange fiber. A 2 g/L lysozyme solution buffered at pH 6.0 flowed through a column charged with a treated or untreated AA-grafted fiber at a space velocity of 20 h −1 . The 10% dynamic binding capacity of the column charged with the former fiber of 200 mg/mL-column was 5.9-fold that of the column charged with the latter fiber, and 2.3-fold that (88 mg/mL-column) of a column charged with CM Sepharose™ Fast Flow (GE Healthcare). Highlights: Acrylic-acid-grafted fibers were post-treated by immersion in water at 353 K. Binding capacities of the fibers for lysozyme were evaluated in a flow-through mode. Dynamic binding capacity (DBC) of the fiber for lysozyme reached 200 mg/mL-bed. The DBC of the post-treated fiber was 5.9-fold that of the untreated fiber.