Novelty in limbal stem cell culture and cell senescence. (April 2019)

• Record Type: Journal Article
• Authors: Boroumand, Nadia; Nosrati Tirkani, Abolfazl; Javid, Dina; Hasani, Ali; Taherzadeh, Danial; Hosseinzadeh, Alireza; Nooripour, Sanaz; Zarei-Ghanavati, Siamak; Hashemy, Seyed Isaac; Alamdari, Daryoush Hamidi
• Is Part Of: Experimental eye research. Volume 181(2019)
• Page Start: 294
• Page End: 301
• Publication Date: 2019-04
• Subjects: Limbus cornea -- Stem cell -- Deficiency -- Amniotic membrane -- Senescence; Ophthalmology -- Periodicals; Eye -- Periodicals; Œil -- Périodiques; Ophthalmology; Periodicals; Electronic journals; 612.8405
• DOI: 10.1016/j.exer.2019.02.015
• Languages: English
• ISSNs: 0014-4835

Abstract

Abstract: Limbal stem cell deficiency is a pathological state. Recently, limbal stem cell (LSC) transplantation has attracted great interest as a therapeutic method which mainly involves in-vitro expansion of LSCs. It is believed that ex-vivo cultivation conditions could affect the outcome of surgery and the rate of successful transplantation. Thus, we aimed to define a suitable culture condition by conducting a research on ex-vivo expanded LSCs to maintain an optimized graft of amniotic membrane with cultivated-limbal stem cells, regarding the quantity and quality, with the hope of improving the clinical outcome. Highlights: Fibrin glue enriched with PRP and growth factors creates 3D scaffold and increases NP63+ cells' number from 3% to around 6%. PRP-fibrin glue attaches the explant on amniotic membrane for 14 days in culture condition without any sign of degradation. Media supplement with N-acetyl cysteine decreases β-galactosidase activity during ex-vivo cell culture.