Single-molecule localization microscopy analysis with ImageJ. (13th March 2019)

• Record Type: Journal Article
• Authors: van de Linde, Sebastian
• Is Part Of: Journal of physics. Volume 52:Number 20(2019)
• Publication Date: 2019-03-13
• Subjects: ImageJ -- Fiji -- image analysis -- super-resolution -- quantitative imaging -- SMLM; Physics -- Periodicals; 530
• DOI: 10.1088/1361-6463/ab092f
• Languages: English
• ISSNs: 0022-3727

Abstract

Abstract: ImageJ is a versatile and powerful tool for quantitative image analysis in microscopy. It is open-source software, platform-independent and enables students and researchers to obtain an easy but thorough introduction into image analysis. Especially the image processing package Fiji is a valuable and powerful extension of ImageJ. Several plugins and macros for single-molecule localization microscopy (SMLM) have been developed during the last decade. These novel tools cover the steps from single-molecule localization and image reconstruction to SMLM data postprocessing such as density analysis, image registration or resolution estimation. This article describes how ImageJ/Fiji can be used for image analysis, reviews existing extensions for SMLM, and aims at introducing and motivating novices and advanced SMLM users alike to explore the possibilities of ImageJ/Fiji for automated and quantitative data analysis.