Selected α-pyrones from the plants Cryptocarya novoguineensis (Lauraceae) and Piper methysticum (Piperaceae) with activity against Haemonchus contortus in vitro. (April 2019)

• Record Type: Journal Article
• Authors: Herath, H.M.P. Dilrukshi; Preston, Sarah; Jabbar, Abdul; Garcia-Bustos, Jose; Addison, Russell S.; Hayes, Sasha; Rali, Topul; Wang, Tao; Koehler, Anson V.; Chang, Bill C.H.; Hofmann, Andreas; Davis, Rohan A.; Gasser, Robin B.
• Is Part Of: International journal for parasitology. Volume 9(2019)
• Page Start: 72
• Page End: 79
• Publication Date: 2019-04
• Subjects: Haemonchus contortus -- Anthelmintic -- Natural products -- Cryptocarya novoguineensis -- Piper methysticum -- α-pyrones; Parasitic diseases -- Chemotherapy -- Periodicals; Drug resistance -- Periodicals; 616.96061
• DOI: 10.1016/j.ijpddr.2018.12.006
• Languages: English
• ISSNs: 2211-3207

Abstract

Abstract: Due to the widespread occurrence and spread of anthelmintic resistance, there is a need to develop new drugs against resistant parasitic nematodes of livestock animals. The Nobel Prize-winning discovery and development of the anti-parasitic drugs avermectin and artemisinin has renewed the interest in exploring natural products as anthelmintics. In the present study, we screened 7500 plant extracts for in vitro-activity against the barber's pole worm, Haemonchus contortus, a highly significant pathogen of ruminants. The anthelmintic extracts from two plants, Cryptocarya novoguineensis and Piper methysticum, were fractionated by high-performance liquid chromatography (HPLC). Subsequently, compounds were purified from fractions with significant biological activity. Four α-pyrones, namely goniothalamin (GNT), dihydrokavain (DHK), desmethoxyyangonin (DMY) and yangonin (YGN), were purified from fractions from the two plants, GNT from C. novoguineensis, and DHK, DMY and YGN (= kavalactones) from P. methysticum . The three kavalactones induced a lethal, eviscerated (Evi) phenotype in treated exsheathed third-stage larvae (xL3s), and DMY and YGN had moderate potencies (IC50 values of 31.7 ± 0.23 μM and 23.7 ± 2.05 μM, respectively) at inhibiting the development of xL3s to fourth-stage larvae (L4s). Although GNT had limited potency (IC50 of 200–300 μM) at inhibiting L4 development, it was the only compound that reduced L4 motility (IC50 of 6.25–12.50 μM). The compounds purified from each plant affected H. contortus in an irreversible manner. These findings suggest that structure-activity relationship studies of α-pyrones should be pursued to assess their potential as anthelmintics. Graphical abstract: Image 1 Highlights: 7500 plant extracts were screened against Haemonchus for anthelmintic activity. Three of these extracts were potent inhibitors of larval motility and/or development. Pure α-pyrones isolated from active fractions exhibited significant nematocidal activity.