Emergence of an Escherichia coli strain co-harbouring mcr-1 and blaNDM-9 from a urinary tract infection in Taiwan. (March 2019)

• Record Type: Journal Article
• Authors: Lin, Yu-Chi; Kuroda, Makoto; Suzuki, Satowa; Mu, Jung-Jung
• Is Part Of: Journal of global antimicrobial resistance. Volume 16(2019)
• Page Start: 286
• Page End: 290
• Publication Date: 2019-03
• Subjects: mcr-1 -- blaNDM-9 -- Escherichia coli; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2018.10.003
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: Analysis of a clinical E. coli strain carrying mcr-1 and bla NDM-9 by whole-genome sequencing. The mcr-1 -carrying plasmid from a human isolate is highly similar to that from a pig isolate. The bla NDM-9 gene may accelerate dissemination through distinct transmission routes. Abstract: Objectives: Multidrug-resistant bacteria have become a serious threat worldwide. In particular, the coexistence of carbapenemase genes and mcr-1 leaves few available treatment options. Here we report a multidrug-resistant Escherichia coli isolate harbouring both mcr-1 and bla NDM-9 from a patient with a urinary tract infection. Methods: Antimicrobial susceptibility and resistance genes of the E. coli isolate were characterised. Furthermore, the assembled genome sequences of mcr-1 - and bla NDM-9 -carrying plasmids were determined and comparative genetic analysis with closely related plasmids was carried out. Results: Three contigs were assembled comprising the E. coli chromosome and two plasmids harbouring mcr-1 (p5CRE51-MCR-1) and bla NDM-9 (p5CRE51-NDM-9), respectively. Whole-genome sequencing revealed that the two antimicrobial resistance genes are located on individual plasmids. Conclusions: The emergence of coexistence of carbapenemase genes and mcr-1 in Enterobacteriaceae highlights a serious threat to antimicrobial therapy.