Steroid hormones in prediction of normal pressure hydrocephalus. Issue 152 (August 2015)

• Record Type: Journal Article
• Authors: Sosvorova, Lucie; Hill, Martin; Mohapl, Milan; Vitku, Jana; Hampl, Richard
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 152(2015)
• Page Start: 124
• Page End: 132
• Publication Date: 2015-08
• Subjects: 7α-OH-DHEA 7α-hydroxy-dehydroepiandrosterone -- 7β-OH-DHEA 7β-hydroxy-dehydroepiandrosterone -- 7-oxo-DHEA 7-oxo-dehydroepiandrosterone -- HSD11B1 11β-hydroxysteroid dehydrogenase type 1 -- HSD11B2 11β-hydroxysteroid dehydrogenase type 2 -- 16α-OH-DHEA 16α-hydroxy-dehydroepiandrosterone -- AD alzheimer's disease -- BBB blood-brain-barrier -- CSF cerebrospinal fluid -- DHEA dehydroepiandrosterone -- DHEAS dehydroepiandrosterone-sulfate -- MR mineralocorticoid receptors -- NPH normal pressure hydrocephalus -- OPLS the method of orthogonal projections to latent structures -- PD Parkinson disease -- UPLC ultra performance liquid chromatography -- VD vascular dementia; Normal pressure hydrocephalus -- Cerebrospinal fluid -- Neuroactive steroids -- Aldosterone -- Prediction; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2015.05.004
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: The laboratory diagnostics of normal pressure hydrocephalus (NPH) is presented. 8 Relevant steroids were determined in cerebrospinal fluid (CSF) in NPH subjects. Prediction model based on selected steroids enabled to differ NPH from controls. Aldosterone in CSF was determined for the first time by instrumental method. Abstract: Normal pressure hydrocephalus (NPH) is a treatable neurological disorder affecting elderly people with the prevalence increasing with age. NPH is caused by abnormal cerebrospinal fluid (CSF) reabsorption and manifested as a balance impairment, urinary incontinence and dementia development. These symptoms are potentially reversible if recognized early. Diagnosis of NPH is difficult and can be easily mistaken for other neurodegenerative disorders, which makes NPH one of the major misdiagnosed diseases worldwide. The aim of the study was to find out the appropriate combination of indicators, based on CSF steroids, which would contribute to a clearer NPH diagnosis. The levels of CSF cortisol, cortisone, dehydroepiandrosterone (DHEA), 7α-OH-DHEA, 7β-OH-DHEA, 7-oxo-DHEA, 16α-OH-DHEA and aldosterone (all LC-MS/MS) were determined in our patients ( n = 30; NPH, 65–80 years) and controls (n = 10; 65–80 years). The model of orthogonal projections to latent structures (OPLS) was constructed to predict NPH. Cortisone, 7α-OH-DHEA, 7β-OH-DHEA, 7-oxo-DHEA, aldosterone, 7α-OH-DHEA /DHEA, 7-oxo-DHEA/7α-OH-DHEA, 7β-OH-DHEA/7-oxo-DHEA and 16α-OH-DHEA/DHEA in the CSF were identified as the key predictors and the model discriminated patients from controls with 100% sensitivity and 100% specificity. The suggested model would contribute to early and accurate NPH diagnosis, enabling promptly treatment of the disease.