Nerve injury induces the expression of syndecan-1 heparan sulfate proteoglycan in primary sensory neurons. (6th August 2015)

• Record Type: Journal Article
• Authors: Murakami, K.; Tanaka, T.; Bando, Y.; Yoshida, S.
• Is Part Of: Neuroscience. Volume 300(2015)
• Page Start: 338
• Page End: 350
• Publication Date: 2015-08-06
• Subjects: ATF-3 activating transcription factor-3 -- cDNA complementary DNA -- CGRP calcitonin gene-related peptide -- CNS central nervous system -- cRNA complementary RNA -- DIG digoxigenin -- DIV day in vitro -- DRG dorsal root ganglion -- ECM extracellular matrix -- FGF2 fibroblast growth factor 2 -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- GFP green fluorescent protein -- HGF hepatocyte growth factor -- HS-GAG heparan sulfate glycosaminoglycan -- HSPG heparan sulfate proteoglycan -- IB4 isolectin B4 (lectin from Bandeiraea simplicifolia) -- IHC immunohistochemistry -- IR immunoreactivity -- mRNA messenger RNA -- PB phosphate buffer -- PBS phosphate-buffered saline -- POD postoperative day -- RT-PCR reverse transcription-polymerase chain reaction -- siRNA small interfering RNA -- SSC standard saline citrate -- TG trigeminal ganglion; syndecan-1 -- heparan sulfate proteoglycan -- nerve injury -- primary sensory neuron -- C-fiber; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2015.05.033
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Syndecan-1 is expressed in injured C-fiber sensory neurons after sciatic nerve axotomy. Syndecan-1 is distributed to injured sciatic nerve fibers after axotomy. Syndecan-1 is distributed to the lamina II of the ipsilateral dorsal horn of the spinal cord after sciatic nerve axotomy. Syndecan-1 is expressed in cranial sensory neurons after axotomy. Syndecan-1 promotes neurite extension in primary cultured DRG neurons. Abstract: Heparan sulfate proteoglycans (HSPGs) have important functions in development of the central nervous system; however, their functions in nerve injury are not yet fully understood. We previously reported the expression of syndecan-1, a type of HSPG, in cranial motor neurons after nerve injury, suggesting the importance of syndecan-1 in the pathology of motor nerve injury. In this study, we examined the expression of syndecan-1, a type of HSPG, in primary sensory neurons after nerve injury in mice. Sciatic nerve axotomy strongly induced the expression of syndecan-1 in a subpopulation of injured dorsal root ganglion (DRG) neurons, which were small in size and had CGRP- or isolectin B4-positive fibers. Syndecan-1 was also distributed in the dorsal horn of the spinal cord ipsilateral to the axotomy, and located on the membrane of axons in lamina II of the dorsal horn. Not only sciatic nerve axotomy, infraorbital nerve axotomy also induced the expression of syndecan-1 in trigeminal ganglion neurons. Moreover, syndecan-1 knockdown in cultured DRG neurons induced a shorter neurite extension. These results suggest that syndecan-1 expression in injured primary sensory neurons may have functional roles in nerve regeneration and synaptic plasticity, resulting in the development of neuropathic pain.