Comparison of Near-Infrared Spectroscopy with Needle Indentation and Histology for the Determination of Cartilage Thickness in the Large Animal Model Sheep. (April 2019)

• Record Type: Journal Article
• Authors: Horbert, Victoria; Lange, Matthias; Reuter, Thomas; Hoffmann, Martin; Bischoff, Sabine; Borowski, Juliane; Schubert, Harald; Driesch, Dominik; Mika, Joerg; Hurschler, Christof; Kinne, Raimund W.
• Is Part Of: Cartilage. Volume 10:Number 2(2019)
• Page Start: 173
• Page End: 185
• Publication Date: 2019-04
• Subjects: near-infrared spectroscopy (NIRS) -- needle indentation -- histology -- articular cartilage thickness -- large animal model; Cartilage -- Periodicals; 616.7
• DOI: 10.1177/1947603517731851
• Languages: English
• ISSNs: 1947-6035

Abstract

The suitability of near-infrared spectroscopy (NIRS) for non-destructive measurement of cartilage thickness was compared with the gold standard needle indentation. A combination of NIRS and biomechanical indentation (NIRS-B) was used to address the influence of varying loads routinely applied for hand-guided NIRS during real-life surgery on the accuracy of NIRS-based thickness prediction. NIRS-B was performed under three different loading conditions in 40 osteochondral cylinders from the load-bearing area of the medial and lateral femur condyle of 20 cadaver joints (left stifle joints; female Merino sheep; 6.1 ± 0.6 years, mean ± standard error of the mean). The cartilage thickness measured by needle indentation within the region analyzed by NIRS-B was then compared with cartilage thickness prediction based on NIRS spectral data using partial least squares regression. NIRS-B repeat measurements yielded highly reproducible values concerning force and absorbance. Separate or combined models for the three loading conditions (the latter simulating load-independent measurements) resulted in models with optimized quality parameters (e.g., coefficients of determination R 2 between 92.3 and 94.7) and a prediction accuracy of < 0.1 mm. NIRS appears well suited to determine cartilage thickness (possibly in a hand-guided, load-independent fashion), as shown by high reproducibility in repeat measurements and excellent reliability compared with tissue-destructive needle indentation. This may provide the basis for non-destructive, intra-operative assessment of cartilage status quo and fine-tuning of repair procedures.