A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W. Issue 13 (6th February 2018)

• Record Type: Journal Article
• Authors: Huang, Biao; Yu, Huixin; Bao, Jiandong; Zhang, Manda; Green, William L; Wu, Sing-Yung
• Is Part Of: Biomarker insights. Volume 2018:Issue 13(2018)
• Publication Date: 2018-02-06
• Subjects: Homogeneous time-resolved fluorescence immunoassay -- pregnancy -- thyroid hormone analogs -- compound W; Biochemical markers -- Periodicals; 572.43
• DOI: 10.1177/1177271918757484
• Languages: English
• ISSNs: 1177-2719

Abstract

Objective: Using compound W (a 3, 3′-diiodothyronine sulfate [T2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.