A rapid non‐target screening method for determining prohibited substances in human urine using liquid chromatography/high‐resolution tandem mass spectrometry. Issue 3 (25th October 2018)

• Record Type: Journal Article
• Authors: Han, Boyoung; Min, Hophil; Jeon, Mijin; Kang, Byeori; Son, Junghyun
• Is Part Of: Drug testing and analysis. Volume 11:Issue 3(2019)
• Page Start: 382
• Page End: 391
• Publication Date: 2018-10-25
• Subjects: doping control -- high‐resolution mass spectrometry -- Non‐target screening method -- open method -- variable data independent acquisition; Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.2495
• Languages: English
• ISSNs: 1942-7603

Abstract

Abstract: Target analysis using liquid chromatography–tandem mass spectrometry is applied for rapidly detecting various prohibited doping substances. Frequent modification is required as additional substances are prohibited. We developed and validated a non‐target screening method requiring no further modification because it analyzes the full spectrum of data in fixed m/z ranges. Urine samples were extracted using solid‐phase extraction and analyzed by employing a method that combines full scan and variable data independent acquisition using high‐resolution mass spectrometry; and all prohibited substances in the urine samples were successfully detected using our screening method. The method was validated in terms of specificity (no interferences), recoveries (29%–131%), matrix effects (35%–237%), limites of detection (0.0002–100 ng/mL), and intra‐ and inter‐day precisions (coefficients of variation lower than 25%). The applicability of this method to doping tests was evaluated by analyzing 14 urine samples. As a result, the non‐target screening method is efficient for conducting anti‐doping tests because it can be applied without any further modification to prohibited drugs as well as to unknown targets that can be prohibited in the future. Abstract : In this study, a total of 111 drugs were analyzed by non‐targeted qualitative analysis using vDIA mode. This approach has the advantage that non‐target substances can be analyzed without changing the condition, in contrast to targeted qualitative analysis. Therefore, it can be used for new drug screening analysis to be added in the future.