Three-dimensional silk impregnated HAp/PHBV nanofibrous scaffolds for bone regeneration. Issue 5 (24th March 2019)

• Record Type: Journal Article
• Authors: Colpankan Gunes, Oylum; Unalan, Irem; Cecen, Berivan; Ziylan Albayrak, Aylin; Havitcioglu, Hasan; Ustun, Ozcan; Ergur, Bekir Ugur
• Is Part Of: International journal of polymeric materials. Volume 68:Issue 5(2019)
• Page Start: 217
• Page End: 228
• Publication Date: 2019-03-24
• Subjects: Bone tissue engineering -- hydroxyapatite -- PHBV -- silk fibroin -- wet-electrospinning; Polymers -- Periodicals; Plastics -- Periodicals; 620.19205
• DOI: 10.1080/00914037.2018.1443928
• Languages: English
• ISSNs: 0091-4037

Abstract

ABSTRACT: Three-dimensional silk fibroin impregnated poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibrous scaffolds with or without hydroxyapatite (HAp) were prepared by wet-electrospinning method followed by freeze-drying. Scaffolds with cotton wool-like structure have the average fiber diameter of 450–850 nm with 80–85% porosity. In-vitro cell culture tests using MG-63 osteosarcoma human cells revealed improved cell viability, alkaline phosphatase (ALP) activity and total cellular protein amount on the silk impregnated scaffolds compared to PHBV and HAp/PHBV scaffolds after 10 days of cell culture. Immunohistochemical analyses on the silk impregnated scaffolds showed that HAp triggered cell penetration and type I collagen production. Besides, HAp mineralization tendency increased with a decrease in percent crystallinity of the scaffolds comprising HAp and silk after 4 weeks of incubation in simulated body fluid. Consequently, cotton wool-like HAp/PHBV-SF scaffold would be a promising candidate as a bone-filling material for tissue regeneration. GRAPHICAL ABSTRACT: