Identification and characterization of a T-2 toxin-producing Fusarium poae strain and the anti-tumor effect of the T-2 toxin on human hepatoma cell line SMMC-7721. Issue 16 (21st March 2019)

• Record Type: Journal Article
• Authors: Zhu, Wenhe; Liu, Lei; Dong, Yuan; Meng, Guixian; Tang, Lu; Li, Yan; Cai, Jianhui; Wang, Huiyan
• Is Part Of: RSC advances. Volume 9:Issue 16(2019)
• Page Start: 9281
• Page End: 9288
• Publication Date: 2019-03-21
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra09967g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : T-2 toxin produced by Fusarium poae strain can induces apoptosis in SMMC-7721 cells. Abstract : T-2 toxin, produced by Fusarium moulds, is a type A trichothecene mycotoxin which is known to inhibit protein synthesis and also reported to induce DNA lesions, potentially causing DNA fragmentation. T-2 toxin is a very potent cytotoxic toxin, which displays anti-tumor properties. Nevertheless, more studies are still needed to explore its antitumor mechanisms as well as its clinical application in cancer treatment. Here, we report the identification and characterization of a T-2 toxin produced by a Fusarium poae isolated from Jilin, Northeast China. 17 strains of Fusarium poae were screened for T-2 toxin-production and one strain with the highest yield was selected further studies. T-2 toxin produced by the selected Fusarium poae was isolated and purified by HPLC. Anticancer properties of the purified T-2 toxin were evaluated with human hepatoma cell SMMC-7721. The purified T-2 toxin inhibits the proliferation of SMMC-7721 cells and induces cell apoptosis. The mitochondrial membrane potential decreased and the intracellular ROS was up-regulated after T-2 treatment of the cells. Further studies revealed that T-2 treatment activates the intrinsic mitochondrial and MAPKs pathway. Our data provide insight into the promising application of the T-2 toxin in cancer treatment.