Fluorescent nitric oxide donor for the detection and killing of Pseudomonas aeruginosa. Issue 12 (11th February 2019)

• Record Type: Journal Article
• Authors: Hibbard, Hailey A. J.; Reynolds, Melissa M.
• Is Part Of: Journal of materials chemistry. Volume 7:Issue 12(2019)
• Page Start: 2009
• Page End: 2018
• Publication Date: 2019-02-11
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb02552e
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : This paper reports a novel fluorescent nitric oxide donor for the simultaneous detection and killing of Pseudomonas aeruginosa . Abstract : The epidemic of multidrug-resistant bacteria calls for the improvement of both detection methods for bacterial infections and methods of treatment. Nitric oxide is a known potent antibacterial agent, but due to its gaseous and highly reactive nature, it is difficult to incorporate into a stable antibacterial compound. In this paper, we synthesize a nitric oxide donor attached to a fluorescent compound, creating a material that can both detect and kill the deadly multi-drug resistant bacteria strain Pseudomonas aeruginosa . Detection occurs through a bacterial enzyme-activated color change, showing a clear and obvious change from blue to yellow under UV light. The synthesized compound spontaneously releases 853 μmol of nitric oxide/g from a 10 mM initial concentration. Antibacterial efficacy studies after exposing Pseudomonas aeruginosa to a 10 mM dose of the synthesized compound show a 55–75% reduction in bacteria after 24 hours. This work is the first instance of a small molecule dual-function material that can both detect and kill bacteria.