MicroRNA-224 impairs adipogenic differentiation of bovine preadipocytes by targeting LPL. (April 2019)

• Record Type: Journal Article
• Authors: Zhang, Yingying; Wang, Yaning; Wang, Hongbao; Ma, Xueyao; Zan, Linsen
• Is Part Of: Molecular and cellular probes. Volume 44(2019)
• Page Start: 29
• Page End: 36
• Publication Date: 2019-04
• Subjects: IMF deposition -- Adipogenic differentiation -- miR-224 -- Bovine preadipocytes -- LPL; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2019.01.005
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. When miR-224 was either overexpressed or silenced, qRT-PCR showed a negative regulatory effect on LPL. mRNA expression levels of the fat-formation-related biomarkers C/EBPα, C/EBPβ, PPARγ, FASN and PLIN1 decreased when miR-224 was overexpressed, while the opposite effect occurred and adipogenic differentiation followed when miR-224 was inhibited. Oil Red O staining. Triglyceride (TG) levels and immunostaining revealed that the accumulation of lipid droplets decreased or increased accordingly. Taken together, our data demonstrated that miR-224 regulated the adipogenic differentiation of bovine preadipocytes by targeting LPL. This provides insight into the molecular basis of IMF deposition in beef cattle. Highlights: miR-224 targets LPL in bovine preadipocytes. Abnormal miR-224 expression resulted in converse expression levels of genes related adipogenic differentiation. miR-214 impairs adipocyte differentiation of bovine pre-adipocytes by targeting LPL.