Long non-coding RNA FGF13-AS1 inhibits glycolysis and stemness properties of breast cancer cells through FGF13-AS1/IGF2BPs/Myc feedback loop. (28th May 2019)

• Record Type: Journal Article
• Authors: Ma, Fei; Liu, Xu; Zhou, Shibo; Li, Wenjie; Liu, Chunxiao; Chadwick, Michelle; Qian, Cheng
• Is Part Of: Cancer letters. Volume 450(2019)
• Page Start: 63
• Page End: 75
• Publication Date: 2019-05-28
• Subjects: Lnc FGF13-AS1 -- Metabolism -- Stemness -- c-Myc; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2019.02.008
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: LncRNAs have been proven to play crucial roles in various processes of breast cancer. LncRNA FGF13-AS1 has been identified as one of the 25 downregulated lncRNAs in breast cancer through analyzing data from two cohorts and TCGA by another group of our lab. In this study, we report that FGF13-AS1 expression is decreased in breast cancer tissue compared with corresponding normal tissue, and the downregulation of FGF13-AS1 is associated with poor prognosis. Functional studies show that FGF13-AS1 inhibits breast cancer cells proliferation, migration, and invasion by impairing glycolysis and stemness properties. Mechanistically, FGF13-AS1 reduces the half-life of c-Myc (Myc) mRNA by binding RNA-binding proteins, insulin-like growth factor 2 mRNA binding proteins (IGF2BPs) and disrupting the interaction between IGF2BPs and Myc mRNA. Furthermore, Myc transcriptionally inhibits FGF13-AS1, forming a feedback loop in this signaling pathway. These results reveal for the first time that FGF13-AS1 functions as a tumor suppressor by inhibiting glycolysis and stemness properties of breast cancer cells, and the FGF13-AS1/IGF2BPs/Myc feedback loop could be a novel therapeutic target for breast cancer patients. Highlights: The expression of FGF13-AS1 is decreased in breast cancer which is associated with poor prognosis. FGF13-AS1 inhibits breast cancer cells proliferation, migration and invasion by impairing glycolysis and stemness properties. FGF13-AS1 reduces the stability of Myc mRNA by directly disrupting the association between Myc mRNA and IGF2BPs. Myc transcriptionally inhibits FGF13-AS1.