Effects of stanniocalcin hormones on rat brown adipose tissue metabolism under fed and fasted conditions. (5th April 2019)

• Record Type: Journal Article
• Authors: Sarapio, Elaine; Souza, Samir Khal; Vogt, Everton Lopes; Rocha, Débora Santos; Fabres, Rafael Bandeira; Trapp, Marcia; Da Silva, Roselis S.M.
• Is Part Of: Molecular and cellular endocrinology. Volume 485(2019)
• Page Start: 81
• Page End: 87
• Publication Date: 2019-04-05
• Subjects: Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2019.02.004
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: In this study we determined the effect of fed and fasting (48 h) states on the expression of stanniocalcin-1 ( Stc1 ) and stanniocalcin-2 ( Stc2 ) in rat brown adipose tissue (BAT), as well as the in vitro effects of human stanniocalcin 1 and 2 (hSTC-1 and hSTC-2) hormones on lipid and glucose metabolism. In addition, lactate, glycogen levels and hexokinase (HK) activity were determined. In fasting Stc2 expression increased markedly. The targets of action of hSTC-1 and hSTC-2 were glucose uptake and oxidation as well as glycogen storage, controlling the energetic metabolism in BAT. The reduction in glycogen concentration induced by hSTC-2 in fed state might have deleterious consequences in BAT, such as decreased thermogenic activity, FA esterification and other adipocyte functions. On the other hand, the increase of glucose uptake caused by hSTC-1 of fed rats could play a role as a plasma glucose-clearing hormone in the postprandial period. Graphical abstract: Image 1 Highlights: BAT expressed Stc1 and Stc2, and fasting increased Stc2 expression. HSTC-1 increased glucose uptake in BAT of fed rats. HSTC-1 increased CO2 formation in BAT of fasting rats. HSTC-2 decreased glycogen levels in BAT of fed rats. HSTC-2 increased glycogen concentration in BAT of fasting rats.