Functional genomic analysis of the human receptive endometrium transcriptome upon administration of mifepristone at the time of follicle rupture. (5th April 2019)

• Record Type: Journal Article
• Authors: Lira-Albarrán, Saúl; Vega, Claudia C.; Durand, Marta; Rangel, Claudia; Larrea, Fernando
• Is Part Of: Molecular and cellular endocrinology. Volume 485(2019)
• Page Start: 88
• Page End: 96
• Publication Date: 2019-04-05
• Subjects: Mifepristone -- Human endometrium -- Differential gene expression -- Selective progesterone receptor modulators; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2019.02.010
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: The aim of this study was to analyze the effects of progesterone withdrawal on gene transcription in receptive endometrium by the administration of a single dose of 50 mg of the anti-progesterone receptor mifepristone (MFP) at the time of follicle rupture (FR). Six volunteer ovulatory women were studied, taking endometrial biopsies of three control and three MFP-treated women on days LH+2 (C-LH+2) and LH+7 (T-MFP), respectively. The biopsies were prepared for RNA isolation or histological and immunohistochemistry studies. The genomic data from 14 women (C-LH+7) were included as a historical control. The functional genomic analysis of the differentially expressed genes showed that MFP interfered negatively with the bio-functions decidualization of uterus and implantation of blastocyst and embryo. The results of this study confirm but also give new information on how MFP affects endometrial gene expression when administered at the time of FR and the dose used in emergency contraception. Highlights: Mifepristone given at the time of FR affects gene markers of endometrial receptivity. Genes involved in endometrial receptivity were down-regulated by MFP. The use of 50 mg of MFP significantly changes endometrial transcriptome. The contraceptive actions of MFP on endometrium might also be mediated by the GR.