Accretion of Dietary Docosahexaenoic Acid in Mouse Tissues Did Not Differ between Its Purified Phospholipid and Triacylglycerol Forms. Issue 1 (29th January 2019)

• Record Type: Journal Article
• Authors: Adkins, Yuriko; Laugero, Kevin D.; Mackey, Bruce; Kelley, Darshan S.
• Is Part Of: Lipids. Volume 54:Issue 1(2019)
• Page Start: 25
• Page End: 37
• Publication Date: 2019-01-29
• Subjects: Adipose tissue -- Brain -- DHA -- Eye -- Heart -- Liver -- n‐3 PUFA -- n‐6 PUFA; Lipids -- Periodicals; Lipids -- Periodicals; Lipiden; Lipides -- Périodiques; 547.77
• DOI: 10.1002/lipd.12115
• Languages: English
• ISSNs: 0024-4201

Abstract

Abstract: Recent studies suggest that dietary krill oil leads to higher omega‐3 polyunsaturated fatty acids (n‐3 PUFA) tissue accretion compared to fish oil because the former is rich in n‐3 PUFA esterified as phospholipids (PL), while n‐3 PUFA in fish oil are primarily esterified as triacylglycerols (TAG). Tissue accretion of the same dietary concentrations of PL‐ and TAG‐docosahexaenoic acid (22:6n‐3) (DHA) has not been compared and was the focus of this study. Mice ( n = 12/group) were fed either a control diet or one of six DHA (1%, 2%, or 4%) as PL‐DHA or TAG‐DHA diets for 4 weeks. Compared with the control, DHA concentration in liver, adipose tissue (AT), heart, and eye, but not brain, were significantly higher in mice consuming either PL‐ or TAG‐DHA, but there was no difference in DHA concentration in all tissues between the PL‐ or TAG‐DHA forms. Consumption of PL‐ and TAG‐DHA at all concentrations significantly elevated eicosapentaenoic acid (20:5n‐3) (EPA) in all tissues when compared with the control group, while docoshexapentaenoic acid (22:5n‐6) (DPA) was significantly higher in all tissues except for the eye and heart. Both DHA forms lowered total omega‐6 polyunsaturated fatty acids (n‐6 PUFA) in all tissues and total monounsaturated fatty acids (MUFA) in the liver and AT; total saturated fatty acid (SFA) were lowered in the liver but elevated in the AT. An increase in the DHA dose, independent of DHA forms, significantly lowered n‐6 PUFA and significantly elevated n‐3 PUFA concentration in all tissues. Our results do not support the claim that the PL form of n‐3 PUFA leads to higher n‐3 PUFA tissue accretion than their TAG form.