Ascorbic acid diminishes bone morphogenetic protein 2‐induced osteogenic differentiation of muscle precursor cells. Issue 4 (6th February 2019)

• Record Type: Journal Article
• Authors: Ferrazzo, Paige C.; Niccoli, Sarah; Khaper, Neelam; Rathbone, Christopher R.; Lees, Simon J.
• Is Part Of: Muscle & nerve. Volume 59:Issue 4(2019)
• Page Start: 501
• Page End: 508
• Publication Date: 2019-02-06
• Subjects: antioxidant -- bone healing -- heterotopic ossification -- myogenesis -- reactive oxygen species -- satellite cell -- skeletal muscle; Neuromuscular diseases -- Periodicals; Muscles -- Periodicals; Nerves -- Periodicals; 616.74
• DOI: 10.1002/mus.26415
• Languages: English
• ISSNs: 0148-639X

Abstract

ABSTRACT: Introduction: Muscle precursor cells (MPC) are integral to the maintenance of skeletal muscle and have recently been implicated in playing a role in bone repair. The primary objective of this study was to understand better the role of oxidative stress during the osteogenic differentiation of MPCs. Methods: Muscle precursor cells were treated with various combinations of ascorbic acid (AA), bone morphogenetic protein (BMP)‐2, and either a superoxide dismutase analog (4‐hydroxy‐TEMPO [TEMPOL]) or polyethyleneglycol‐conjugated catalase. Muscle precursor cell proliferation and differentiation were determined, and alkaline phosphatase activity was measured as an index of osteogenic differentiation. Results: After treatment with 200 μM AA, superoxide was increased 1.5‐fold, whereas AA in combination with 100 ng/ml BMP‐2 did not increase alkaline phosphatase (ALP) activity. When cells were treated with TEMPOL in combination with 100 ng/ml BMP‐2 and 200 μM AA, ALP activity significantly increased. Discussion: These data suggest that increasing oxidative stress with AA induces sublethal oxidative stress that prevents BMP‐2‐induced osteogenic differentiation of MPCs. Muscle Nerve 59:501–508, 2019