Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA. Issue 7 (23rd November 2018)

• Record Type: Journal Article
• Authors: Patwardhan, Neeraj N.; Cai, Zhengguo; Newson, Colby N.; Hargrove, Amanda E.
• Is Part Of: Organic & biomolecular chemistry. Volume 17:Issue 7(2019)
• Page Start: 1778
• Page End: 1786
• Publication Date: 2018-11-23
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c8ob02467g
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : A fluorescent peptide displacement assay combined with statistical analysis is used for screening small molecules against different RNA targets and profiling their affinity/selectivity patterns. Abstract : A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.