An in vitro model to evaluate the impact of environmental fine particles (PM0.3-2.5) on skin damage. (1st May 2019)

• Record Type: Journal Article
• Authors: Verdin, Anthony; Cazier, Fabrice; Fitoussi, Richard; Blanchet, Natacha; Vié, Katell; Courcot, Dominique; Momas, Isabelle; Seta, Nathalie; Achard, Sophie
• Is Part Of: Toxicology letters. Volume 305(2019)
• Page Start: 94
• Page End: 102
• Publication Date: 2019-05-01
• Subjects: 4-HNE 4-hydroxynonenal -- BSA bovine serum albumin -- DAPI 4', 6-diamidino-2-phenylindole dihydrochloride -- PM particulate matter -- H&E haematoxylin and eosin -- MMP metalloproteinase -- PBS phosphate buffered saline -- RHE Reconstructed Human Epidermis -- RT-qPCR real-time quantitative PCR; Airborne fine particles -- In vitro skin model -- Inflammatory response -- Oxidative stress -- Premature skin ageing; Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2019.01.016
• Languages: English
• ISSNs: 0378-4274

Abstract

Highlights: Airborne particles induce skin damage. Oxidative and inflammatory pathways modulation after skin exposure to PM. Premature skin ageing after PM exposure. Abstract: Exposure to airborne particulate matter (PM) has significant effects on human health mainly leading to cardio-respiratory diseases. However very few data are available regarding the impact of PM on the skin, so to better understand the impact of fine particle (PM0.3-2.5 ) on both inflammatory response and epidermal structure, we exposed a reconstructed human epidermis (RHE) to several doses of PM collected in Cotonou (Benin, West Africa). After 24 h of exposure, inflammatory response, histological observations, and gene expression related to oxidative stress, antioxidant defense and structural damages were determined. No PM-linked changes in tissue morphology or membrane integrity were observable. PM was however cytotoxic in a dose dependent manner. An inflammatory response appeared as shown by the increase in IL-1α and IL-8 cytokine productions. PM also induced oxidative stress, leading to an increase in 4-HNE immunostaining and to the up-regulation of HMOX1, MT1G and MT1E . Finally, PM had a negative impact on fundamental skin functions such as tissue anchorage, cell differentiation, cornification / skin desquamation and apoptosis. Our data show that airborne fine particles have an adverse effect on skin integrity, most probably leading to accelerated ageing.