Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells. Issue 2 (1st February 2019)

• Record Type: Journal Article
• Authors: Zhao, Jia-Yu; Wang, Jia-Qi; Wu, Li; Zhang, Feng; Chen, Zhi-Peng; Li, Wei-Dong; Cai, Hao; Liu, Xiao
• Is Part Of: Journal of Asian natural products research. Volume 21:Issue 2(2019)
• Page Start: 186
• Page End: 195
• Publication Date: 2019-02-01
• Subjects: Rhubarb -- emodin -- acute pancreatitis -- ROS -- cytokine; Materia medica -- Asia -- Periodicals; Pharmacognosy -- Asia -- Periodicals; Medicinal plants -- Asia -- Periodicals; Medicine, Oriental -- Asia -- Periodicals; 615.321095
• DOI: 10.1080/10286020.2017.1408594
• Languages: English
• ISSNs: 1028-6020

Abstract

Abstract: The study was intended to investigate the protective effects of emodin against cell injury and inflammation in AR42J cells. We determined trypsin and lipase activity, intracellular ROS and MMP using specific assay kits. The related protein expression and TNF-α and IL-6 in the medium were assayed by Western blot and ELISA kits. Results showed that emodin could protect AR42J cells against cell injury caused by cerulein and lipopolysaccharide which were possibly associated with inhibition of mitochondrial damage, ROS production, and then significantly inhibited ROS-mediated pathway, and ameliorated pancreatic cells injury by depleting the levels of inflammatory cytokines. Abstract :