Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule. Issue 12 (14th March 2019)

• Record Type: Journal Article
• Authors: Fry, Lindsay M.; Bastos, Reginaldo G.; Stone, Brad C.; Williams, Laura B.; Knowles, Donald P.; Murphy, Sean C.
• Is Part Of: Vaccine. Volume 37:Issue 12(2019)
• Page Start: 1546
• Page End: 1553
• Publication Date: 2019-03-14
• Subjects: BAEC bovine aortic endothelial cells -- BoLA bovine leukocyte antigen -- CBC complete blood count -- CO codon-optimized -- ECF east coast fever -- ELISA enzyme linked immunosorbent assay -- GG gene gun -- HEK human embryonic kidney cells -- IFNγ interferon gamma -- ITM infection and treatment method -- MHC major histocompatibility complex -- PIM polymorphic immunodominant molecule; DNA vaccination -- East Coast Fever -- Gene gun immunization -- Cattle -- Vaccine; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2019.02.009
• Languages: English
• ISSNs: 0264-410X

Abstract

Abstract: Theileria parva kills over one million cattle annually in sub-Saharan Africa. Parasite genetic complexity, cellular response immunodominance, and bovine MHC diversity have precluded traditional vaccine development. One potential solution is gene gun (GG) immunization, which enables simultaneous administration of one or more DNA-encoded antigens. Although promising in murine, porcine, and human vaccination trials, bovine GG immunization studies are limited. We utilized the model T. parva antigen, polymorphic immunodominant molecule (PIM) to test bovine GG immunization. GG immunization using a mammalian codon optimized PIM sequence elicited significant anti-PIM antibody and cell-mediated responses in 7/8 steers, but there was no difference between immunized and control animals following T. parva challenge. The results suggest immunization with PIM, as delivered here, is insufficient to protect cattle from T. parva . Nonetheless, the robust immune responses elicited against this model antigen suggest GG immunization is a promising vaccine platform for T. parva and other bovine pathogens.