Evaluation of polyherbal ayurvedic formulation 'Peedantak Vati' for anti-inflammatory and analgesic properties. (10th May 2019)

• Record Type: Journal Article
• Authors: Balkrishna, Acharya; Ranjan, Ravikant; Sakat, Sachin S.; Sharma, Vinay K.; Shukla, Ravikant; Joshi, Khemraj; Devkar, Raviraj; Sharma, Niti; Saklani, Sonia; Pathak, Prateek; Kumari, Pratima; Agarwal, Veena R.
• Is Part Of: Journal of ethnopharmacology. Volume 235(2019)
• Page Start: 361
• Page End: 374
• Publication Date: 2019-05-10
• Subjects: NO Nitric oxide -- TNF-α Tumor necrosis factor-α -- IL-6 Interleukins-6 -- IL-1β Interleukins-1beta -- PGs Prostaglandins -- COX Cyclooxygenase -- LPS lipopolysaccharides -- ELISA Enzyme-linked immunosorbent assay -- MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) -- DMEM Dulbecco's Modified Eagle Medium -- RPMI Roswell Park Memorial Institute 1640 Medium -- FBS Fetal Bovine Serum -- PMA Phorbol 12-myristate 13-acetate -- NSAIDs Non steroidal anti-inflammatory drugs -- PV Peedantak Vati -- CPCSEA Committee for the Purpose of Control And Supervision of Experiments on Animals -- IAEC Institutional Animal Ethical Committee -- AYUSH Ministry of Ayurveda, Yoga & Naturopathy, Unani, Siddha and Homoeopathy -- ANOVA A one-way analysis of variance -- TED Therapeutic equivalent dose -- HD High dose -- MPE maximum possible effect -- LT Latency time -- INDO Indomethacin -- NC normal control -- HPLC High Performance Liquid Chromatography -- LOD Limit of detections -- LOQ Limit of quantification -- HPTLC High-Performance Thin-Layer Chromatography, Liquid chromatography–mass spectrometry -- API The Ayurvedic Pharmacopoeia of India -- AFI Ayurvedic Formulary of India -- LC-MS/MS Liquid chromatography-tandem mass spectrometry -- ICP-MS Inductively coupled plasma mass spectrometry; Peedantak Vati -- Inflammation -- Analgesic -- Edema -- Nitric oxide -- RAW264.7 -- THP-1 -- HPLC -- HPTLC -- LC-MS/MS; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2019.01.028
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Peedantak Vati (PV) is a polyherbal ayurvedic formulation, which is regularly prescribed by the ayurvedic practitioner for the inflammatory disorders and joints pain in India. It is composed of 23 different herbs and minerals, described in ayurvedic text for their anti-inflammatory and analgesic properties. Aim of the study: To investigate anti-inflammatory and anti-nociceptive potential of 'Peedantak Vati' using in vitro and in vivo methods. Materials and methods: In-vitro anti-inflammatory activity of PV was studied by estimating nitric oxide (NO) and LPS-induced pro-inflammatory cytokines IL-6 and TNF-α, using murine macrophage RAW264.7 and human monocyte THP-1 cell lines. PV's anti-inflammatory potential was studied in vivo using carrageenan-induced rat paw edema model. Similarly, anti-nociceptive property of PV was evaluated using hot plate, tail flick, formalin and writhing tests on CD-1 mice. Phytochemical profiling of hydro-alcoholic extract of PV was done using HPLC and HPTLC techniques to identify different marker compounds. These identified marker compounds were confirmed using LC-MS/MS analysis. Results: In vitro results strongly suggest that, PV significantly (p < 0.001) inhibited NO release and LPS-stimulated pro-inflammatory cytokines IL-6 and TNF-α, in murine RAW264.7 and human THP-1 cells. Further, PV demonstrated significant (p < 0.05) anti-inflammatory activity at different time points after carrageenan injection with maximum effect at 2 h (40.4 ± 5.2% at 400 mg/kg). Similarly, PV significantly (p < 0.05) decreased nociceptive pain, studied using hot plate, tail flick, formalin and writhing tests. Moreover, HPLC and HPTLC methods were developed for the standardization of PV. Five marker phytocompounds viz. rutin, caffeic acid, colchicine, withaferin A and curcumin were identified and quantified by HPLC and HPTLC methods. The presence of these phytoconstituents was confirmed by LC-MS/MS analysis. Conclusion: The findings of the study strongly suggest that, the polyherbal ayurvedic formulation 'Peedantak Vati' possesses remarkable anti-inflammatory and analgesic property, providing potent alternative for currently available allopathic medicines such as non steroidal anti-inflammatory drugs (NSAIDs). Graphical abstract: fx1