Evaluation of bisphenol A exposure induced oxidative RNA damage by liquid chromatography-mass spectrometry. (May 2019)

• Record Type: Journal Article
• Authors: Li, Shangfu; Jin, Yibao; Zhao, Hongzhi; Jiang, Yuyang; Cai, Zongwei
• Is Part Of: Chemosphere. Volume 222(2019)
• Page Start: 235
• Page End: 242
• Publication Date: 2019-05
• Subjects: Bisphenol A -- RNA damage -- Modified nucleosides -- Toxicity -- Liquid chromatography-mass spectrometry -- Parallel reaction monitoring; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.01.120
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Highlighted evidence suggests the possible implication of bisphenol A (BPA) exposure on a variety of biological functions, such as DNA damage. Similar to DNA, exposed to BPA may also have potential risks to RNA damage due to its induction of reactive oxygen species. However, there are no related research reports about such health risks of BPA. Therefore, this work tried to investigate the BPA exposure induced oxidative RNA damage by detecting urinary nucleosides, the end-products of RNA metabolism. An ultra-high performance liquid chromatography-Orbitrap mass spectrometry method was applied to selectively and sensitively determine urinary nucleosides. As a result, 66 nucleosides were identified and the effects of BPA exposure on these nucleosides in rat urine samples were evaluated. The nucleosides showed different changing tendency along with different exposure dose of BPA. The strongest effect was observed in high does-exposure rats, indicating dose-response relationship between BPA-treatment and urinary nucleosides. Significant change of some nucleosides, including 8-oxoguanosine, was observed in the high-dose exposure group, suggesting obvious RNA damage to rats. To the best of our knowledge, it is the first study about the RNA damage induced by BPA exposure. The results provided a new perspective on the toxic effects of BPA exposure. Graphical abstract: Image 1 Highlights: The relationship of BPA exposure and oxidative RNA damage was firstly investigated. RNA damage induced by BPA was studied by an isotope labeling approach. The influence of BPA exposure on the urinary nucleosides was dose-response.