Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell 'stemness'. Issue 1 (1st February 2019)

• Record Type: Journal Article
• Authors: Svoradová, Andrea; Vašíček, Jaromír; Ostró, Alexander; Chrenek, Peter
• Is Part Of: Zygote. Volume 27:Issue 1(2019)
• Page Start: 46
• Page End: 48
• Publication Date: 2019-02-01
• Subjects: ALDEFLUOR, -- ALDH, -- Chicken, -- Flow cytometry, -- Primordial germ cells; Developmental biology -- Periodicals; Embryology -- Periodicals; Zygotes -- Periodicals; Gametes -- Periodicals; 571.864
• DOI: 10.1017/S0967199418000631
• Languages: English
• ISSNs: 0967-1994

Abstract

Summary: Chicken primordial germ cells (PGCs) are the primary pluripotent stem cell types that will differentiate towards germ cells. High aldehyde dehydrogenase (ALDH) activity is considered as a functional marker for the detection of cell 'stemness'. In our study the ALDEFLUOR™ kit was used for determination of ALDH activity in PGCs. PGCs were co-stained with diethylaminobenzaldehyde (DEAB) and ALDH and analyzed by flow cytometry. Our results showed a small cell population (8.0 ± 3.3%) upon preincubation of the cells with the specific inhibitor DEAB, however cells without inhibitor staining showed a fluorescence shift as an ALDH-positive population (70.5 ± 1.6%). These findings indicate higher expression of ALDH in PGCs and ALDH activity can therefore be used as a new functional marker for the detection of cell 'stemness' in chicken PGCs. These results may have importance for characterization of PGCs as a potential genetic resource in poultry. Further research is necessary to elucidate the role of this functional marker in these cells.