A novel miRNA, miR-13664, targets CpCYP314A1 to regulate deltamethrin resistance in Culex pipiens pallens. Issue 2 (3rd July 2018)

• Record Type: Journal Article
• Authors: Sun, X. H.; Xu, N.; Xu, Y.; Zhou, D.; Sun, Y.; Wang, W. J.; Ma, L.; Zhu, C. L.; Shen, B.
• Is Part Of: Parasitology. Volume 146:Issue 2(2019)
• Page Start: 197
• Page End: 205
• Publication Date: 2018-07-03
• Subjects: Culex pipiens pallens, -- deltamethrin resistance, -- CpCYP314A1, -- miR-13664; Parasitology -- Periodicals; Electronic journals; 616.96
• DOI: 10.1017/S0031182018001002
• Languages: English
• ISSNs: 0031-1820

Abstract

Abstract: Extensive insecticide use has led to the resistance of mosquitoes to these insecticides, posing a major barrier to mosquito control. Previous Solexa high-throughput sequencing of Culex pipiens pallens in the laboratory has revealed that the abundance of a novel microRNA (miRNA), miR-13664, was higher in a deltamethrin-sensitive (DS) strain than a deltamethrin-resistant (DR) strain. Real-time quantitative PCR revealed that the miR-13664 transcript level was lower in the DR strain than in the DS strain. MiR-13664 oversupply in the DR strain increased the susceptibility of these mosquitoes to deltamethrin, whereas inhibition of miR-13664 made the DS strain more resistant to deltamethrin. Results of bioinformatic analysis, quantitative reverse-transcriptase polymerase chain reaction, luciferase assay and miR mimic/inhibitor microinjection revealed CpCYP314A1 to be a target of miR-13664. In addition, downregulation of CpCYP314A1 expression in the DR strain reduced the resistance of mosquitoes to deltamethrin. Taken together, our results indicate that miR-13664 could regulate deltamethrin resistance by interacting with CpCYP314A1, providing new insights into mosquito resistance mechanisms.