Variability of Spleen and Mesenteric Lymph Node in Control Cynomolgus Monkeys (Macaca fascicularis) from Nonclinical Safety Studies: A Retrospective Assessment. (January 2019)

• Record Type: Journal Article
• Authors: Everds, Nancy E.; Reindel, James; Werner, Jonathan; Craven, W. A.
• Is Part Of: Toxicologic pathology. Volume 47:Number 1(2019)
• Page Start: 53
• Page End: 72
• Publication Date: 2019-01
• Subjects: immune system -- immunotoxicity -- nonhuman primate -- spleen -- lymph node -- organ weight -- cynomolgus monkey; Pathology -- Periodicals; Toxicology -- Periodicals; Pathology; Toxicology; 615.9
• DOI: 10.1177/0192623318809073
• Languages: English
• ISSNs: 0192-6233

Abstract

We assessed the variability of spleen and mesenteric lymph node (MLN) microscopic observations and the correlations of these observations with other study data from 478 control cynomolgus monkeys from 53 routine nonclinical safety studies. Spleen weight parameters (absolute and relative to body or brain weights) were highly variable both within a control group on an individual study (up to 5.11-fold) and among animals with the same light microscopic observation. Grades for microscopic observations were also highly variable. The most frequent microscopic observations for spleen were changes in the size and number of germinal centers (58%), acidophilic (hyaline) material in lymphoid follicles (52%), and compound lymphoid follicles (20%). The most frequent microscopic observations in the MLN were eosinophil infiltrates (90%), changes in size and number of germinal centers (42%), and brown pigment (21%). The only meaningful relationships ( r 2 > 0.3) were positive correlations between reticuloendothelial hyperplasia and malarial pigment in the spleen and between each of these observations and spleen weight parameters. We conclude that determination of test article–related effects on the immune system in routine monkey toxicology studies requires careful consideration and a weight-of-evidence approach due to the low numbers of animals/group, the inherent variability in spleen and MLN parameters, and the infrequent correlation among immune system–related end points.