Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor. (1st May 2019)

• Record Type: Journal Article
• Authors: Pino, Marcela Sofía; Rodríguez-Jasso, Rosa M.; Michelin, Michele; Ruiz, Héctor A.
• Is Part Of: Carbohydrate polymers. Volume 211(2019)
• Page Start: 349
• Page End: 359
• Publication Date: 2019-05-01
• Subjects: Glucan -- Glucose -- Biofuels -- Autohydrolysis -- Severity factor -- Biorefinery; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.01.111
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: HT produces a cellulose rich solid with high susceptibility to enzymatic attack. EH efficiency is negatively affected by solid loading increase. Cellulases undergo rapid activity lose during the first hours of saccharification. Horizontal bioreactors improve glucose production at high solid loadings. Abstract: One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194 °C/30 min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6 g/L at 72 h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.