Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb. seeds. (1st April 2019)

• Record Type: Journal Article
• Authors: Zhang, Wen-Na; Su, Ri-Na; Gong, Li-Li; Yang, Wei-Wei; Chen, Juan; Yang, Rui; Wang, Ya; Pan, Wen-Juan; Lu, Yong-Ming; Chen, Yan
• Is Part Of: Carbohydrate polymers. Volume 209(2019)
• Page Start: 363
• Page End: 371
• Publication Date: 2019-04-01
• Subjects: D-glucose (PubChem CID: 5793) -- D-galactose (PubChem CID: 6036) -- D-mannose (PubChem CID: 18950) -- D-arabinose (PubChem CID: 229) -- Glucuronic acid (PubChem CID: 94715) -- Trifluoroacetic acid (PubChem CID: 6422) -- Pyridine (PubChem CID: 1049) -- Acetic anhydride (PubChem CID: 7918) -- Sodium hydroxide (PubChem CID: 14798) -- Methyl iodide (PubChem CID: 6328) -- Dimethyl sulfoxide (PubChem CID: 679) -- 1-phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051) -- Sodium borohydride (PubChem CID: 4311764); Euryale feroxseed -- Glucan -- Hypoglycemic activity -- Insulin resistance -- HepG2 cells -- 3T3-L1 cells; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.01.044
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: A new glucan EFSP-1 was firstly obtained from the seeds of Euryale ferox Salisb. Structure of EFSP-1 was characterized by FT-IR, GC–MS and NMR. EFSP-1 possessed high hypoglycemic activities in vitro . EFSP-1 could improve insulin resistant through PI3K/Akt signal pathway in IR HepG2 cells. Abstract: In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb. by DEAE sepharose FF and Superdex ™ 75 gel chromatography. The average molecular weight (Mw) of EFSP-1 was 8.75 kDa. Monosaccharides composition analysis indicated that EFSP-1 was a glucan. The structure of EFSP-1 was characterized by analysis of FT-IR, GC–MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)- α -D-Glc p with branches substituted at O-6 and terminated with T- α -D-Glc p . Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells. The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells. Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.