PGS Scaffolds Promote the In Vivo Survival and Directional Differentiation of Bone Marrow Mesenchymal Stem Cells Restoring the Morphology and Function of Wounded Rat Uterus. Issue 5 (7th February 2019)

• Record Type: Journal Article
• Authors: Xiao, Bang; Yang, Wenjun; Lei, Dong; Huang, Jinfeng; Yin, Yupeng; Zhu, Yiqing; You, Zhengwei; Wang, Fang; Sun, Shuhan
• Is Part Of: Advanced healthcare materials. Volume 8:Issue 5(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-02-07
• Subjects: bone marrow mesenchymal stem cells -- endometrium regeneration -- intrauterine adhesion -- poly(glycerol sebacate) scaffolds; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201801455
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: Intrauterine adhesion (IUA) causing infertility and recurrent miscarriage of reproductive female mammals usually results from endometrium injury. Nevertheless, there is no efficient therapeutic method to avoid IUA. Bone marrow derived mesenchymal stem cells (BMSCs) are an important cell source for tissue regeneration. This study designs and explores the ability of BMSC‐loaded elastic poly(glycerol sebacate) (PGS) scaffold to prevent IUA and compares the effect of PGS with poly(lactic‐co‐glycolic acid) (PLGA) and collagen scaffolds in resumption of damaged rat uteruses. The 3D architecture provided by PGS scaffolds favors the attachment and growth of rat BMSCs. In vivo bioluminescence imaging shows that compared with direct BMSC intrauterine injection, PLGA, and collagen scaffolds, the PGS scaffold significantly prolongs the retention time of BMSCs in a wounded rat uterus model. More importantly, BMSCs can directly differentiate into endometrial stromal cells after transplantation of PGS/BMSCs constructs, but not PLGA/BMSCs and collagen/BMSCs. It is found that the level of transforming growth factor β1 (TGF‐β1), basic fibroblast growth factor (bFGF), vascular endothelial growth factor, and insulin‐like growth factors in the injured endometrium adjacent to PGS/BMSCs constructs is higher than those of rats receiving PLGA/BMSCs, collagen/BMSCs, or BMSCs intrauterine transplantation. Besides, transplantation of PGS/BMSCs leads to better morphology recovery of the damaged uterus than PLGA/BMSCs and collagen/BMSCs. The receptive fertility of PGS/BMSCs is 72.2 ± 6.4%, similar to the one of collagen/BMSCs, but significantly higher than 42.3 ± 3.9% in PLGA/BMSCs. Taken together, PGS/BMSCs may be a promising candidate for preventing IUA. Abstract : Poly(glycerol sebacate) (PGS) scaffolds show a modulus similar to the one of native rat uterus tissue. PGS scaffolds can significantly prolong the retention of the bone marrow derived mesenchymal stem cells (BMSCs) in an injured rat uterus model. More importantly, BMSCs in the PGS/BMSCs constructs can directly differentiate into endometrial stromal cells. Finally, PGS/BMSCs significantly promote the morphologic and functional recovery of the injured rat uterus.