Overexpression of CONSTANS-like 16 enhances chlorophyll accumulation in petunia corollas. (March 2019)

• Record Type: Journal Article
• Authors: Ohmiya, Akemi; Oda-Yamamizo, Chihiro; Kishimoto, Sanae
• Is Part Of: Plant science. Volume 280(2019)
• Page Start: 90
• Page End: 96
• Publication Date: 2019-03
• Subjects: ARC5/6 ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5/6 -- CAO chlorophyllide a oxygenase -- CHLG chlorophyll synthase -- CHLH Mg-chelatase subunit H -- CHLM Mg-protoporphyrin IX methyltransferase -- COL16 CONSTANS-like 16 -- CRD Mg-protoporphyrin IX monomethylester cyclase -- FBP2 FLORAL BINDING PROTEIN 2 -- GLK GOLDEN2-LIKE -- HEMA glutamyl-tRNA reductase -- HY5 LONG HYPOCOTYL 5 -- PaO pheophorbide a oxygenase -- PDV1 PLASTID DIVISION 1 -- POR protochlorophyllide oxidoreductase -- PPH pheophytinase -- RCCR red chlorophyll catabolite reductase -- SGR STAY GREEN; CONSTANS-like 16 -- Chlorophyll biosynthesis -- Transcription factor -- Petunia hybrida -- Gene expression; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2018.11.013
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: PhCOL16 expression is associate with chlorophyll content in petunia cultivars. PhCOL16 overexpression in petunia increases chlorophyll level in corollas. PhCOL16 overexpression increases chlorophyll biosynthesis gene expression. Abstract: We have previously found that a gene closely related to Arabidopsis CONSTANS-like 16 ( COL16 ) was coordinately expressed with chlorophyll content in chrysanthemum petals and leaves. Here, to elucidate whether COL16 is involved in the regulation of chlorophyll biosynthesis and accumulation, we analyzed the function of COL16 in petunia ( Petunia hybrida ). We identified three petunia COL16 homologs: PhCOL16a, PhCOL16b, and PhCOL16c . Expression patterns of all three homologs were associated with chlorophyll content, with lower levels in white corollas than in pale green corollas, and relatively high levels in leaves. The result suggests that PhCOL16 homologs are involved in chlorophyll accumulation. We introduced a PhCOL16a overexpression construct into petunia. The transgenic plants had pale green corollas with a higher chlorophyll content than wild-type plants. Expression of genes encoding key enzymes of chlorophyll biosynthesis was significantly higher in the transgenic plants than in the wild-type plants. The results indicate that PhCOL16 positively regulates chlorophyll biosynthesis.