Activation of plasma membrane H+-ATPases participates in dormancy alleviation in sunflower seeds. (March 2019)

• Record Type: Journal Article
• Authors: De Bont, Linda; Naim, Elissa; Arbelet-Bonnin, Delphine; Xia, Qiong; Palm, Emily; Meimoun, Patrice; Mancuso, Stefano; El-Maarouf-Bouteau, Hayat; Bouteau, François
• Is Part Of: Plant science. Volume 280(2019)
• Page Start: 408
• Page End: 415
• Publication Date: 2019-03
• Subjects: Dormancy -- H+-ATPase -- Hormones -- Reactive oxygen species -- Seeds -- Sunflower; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2018.12.015
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Germination of seeds is a critical step in agriculture. Dormant and non-dormant seed cells present different polarization due to the regulation of plasma membrane H + -ATPase. ABA dependent inhibition of H + -ATPases could participate in dormancy maintenance. Ethylene-and ROS-dependent H + -ATPase stimulation could participate in dormancy release. Abstract: Using various inhibitors and scavengers we took advantage of the size of sunflower ( Helianthus annuus ) seeds to investigate in vivo the effects of hormones, namely abscisic acid (ABA) and ethylene (ET), and reactive oxygen species (ROS) on the polarization of dormant (D) and non-dormant (ND) embryonic seed cells using microelectrodes. Our data show that D and ND seed cells present different polarization likely due to the regulation of plasma membrane (PM) H + -ATPase activity. The data obtained after addition of hormones or ROS scavengers further suggest that ABA dependent inhibition of PM H + -ATPases could participate in dormancy maintenance and that ET-and ROS-dependent PM H + -ATPase stimulation could participate in dormancy release in sunflower seeds.