Intestinal epithelial cell apoptosis due to a hemolytic toxin from Vibrio vulnificus and protection by a 36 kDa glycoprotein from Rhus verniciflua Stokes. (March 2019)

• Record Type: Journal Article
• Authors: Lee, Young-Min; Park, Jong Pil; Lim, Kye-Taek; Lee, Sei-Jung
• Is Part Of: Food and chemical toxicology. Volume 125(2019)
• Page Start: 46
• Page End: 54
• Publication Date: 2019-03
• Subjects: Vibrio vulnificus -- VvhA -- Rhus verniciflua Stokes -- Caspase-3 -- Apoptosis -- Gastrointestinal epithelial cells; Bax Bcl-2 associated X protein -- CM-H2DCFDA 2′, 7′ -dichlorofluorescein diacetate -- IPTG isopropyl-β-dthiogalactopyranoside -- JNK c-Jun N-terminal kinase -- MAPKs mitogen-activated protein kinases -- NAC N-acetyl-L-cysteine -- NF-κB nuclear factor-kappa B -- PKC phosphorylation of protein kinase C -- PMSF phenylmethylsulfonyl fluoride -- PVDF polyvinylidene fluoride -- ROS reactive oxygen species -- RVS Rhus verniciflua stokes -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- V. vulnificus Vibrio vulnificus; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2018.12.041
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Rhus verniciflua stokes (RVS) has been used as a functional food to cure inflammatory diseases in Korea. In the present study, we carry out an investigation of the cellular mechanism of a 36 kDa glycoprotein isolated from RVS fruit (RVS glycoprotein) during the apoptosis of human gastrointestinal epithelial HCT116 cells induced by the hemolytic toxin (VvhA) produced by V. vulnificus . Recombinant protein (r) VvhA produced by V. vulnificus stimulated apoptosis by activating the phosphorylation of protein kinase C (PKC) through the production of intracellular reactive oxygen species (ROS). However, RVS glycoprotein significantly inhibited the level of ROS production and PKC activation in rVvhA-stimulated HCT116 cells. Interestingly, we found that RVS glycoprotein has inhibitory effects on the phosphorylation of c-Jun N-terminal kinase (JNK) and nuclear factor-kappa B (NF-κB), which are responsible for the expression of Bax and cleaved caspase-3 in HCT116 cells treated with rVvhA, respectively. On the basis of these results, we suggest that RVS glycoprotein blocks mitochondrial apoptotic cell death induced by rVvhA via the inhibition of ROS-mediated signaling events in HCT116 cells. Highlights: RVS glycoprotein blocks cell death induced by V. vulnificus, VvhA. RVS glycoprotein reduces ROS production to recover cell death induced by rVvhA. RVS glycoprotein suppresses PKC/JNK/NF-κB pathway activated by rVvhA. RVS glycoprotein inhibits expression of Bax and cleaved-caspase 3 induced by rVvhA.