Gene Delivery: Inhaled Nanoformulated mRNA Polyplexes for Protein Production in Lung Epithelium (Adv. Mater. 8/2019). Issue 8 (19th February 2019)

• Record Type: Journal Article
• Authors: Patel, Asha Kumari; Kaczmarek, James C.; Bose, Suman; Kauffman, Kevin J.; Mir, Faryal; Heartlein, Michael W.; DeRosa, Frank; Langer, Robert; Anderson, Daniel G.
• Is Part Of: Advanced materials. Volume 31:Issue 8(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-02-19
• Subjects: biomaterials -- gene delivery -- inhalation -- messenger RNA -- topology; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adma.201970053
• Languages: English
• ISSNs: 0935-9648

Abstract

Abstract : In vitro transcribed messenger RNA is nebulized using poly(beta amino esters) for inhalable delivery to the lung as described by Daniel G. Anderson and co‐workers in article number1805116 . Hyperbranched polymers are synthesized, which form stable mRNA‐polyplexes that distribute uniformly throughout the lungs of mice and are translated to reporter protein in the lung epithelium.