HPLC with cellulose Tris (3, 5‐DimethylPhenylcarbamate) chiral stationary phase: Influence of coating times and coating amount on chiral discrimination. Issue 3 (11th January 2019)

• Record Type: Journal Article
• Authors: Wei, Qiuhong; Su, Hongjiu; Gao, Diannan; Wang, Shudong
• Is Part Of: Chirality. Volume 31:Issue 3(2019)
• Page Start: 164
• Page End: 173
• Publication Date: 2019-01-11
• Subjects: cellulose tris (3, 5‐dimethylphenylcarbamate) -- chiral recognition -- chiral stationary phase -- enantioseparation -- high‐performance liquid chromatography -- resolution; Chirality -- Periodicals; Pharmaceutical chemistry -- Periodicals; 541.22
• DOI: 10.1002/chir.23044
• Languages: English
• ISSNs: 0899-0042

Abstract

Abstract: Coating cellulose tris (3, 5‐dimethylphenylcarbamate) (CDMPC) on silica gels with large pores have been demonstrated as an efficient way for the preparation of chiral stationary phase (CSP) for high‐performance liquid chromatography (HPLC). During the process, a number of parameters, including the type of coating solvent, amount of coating, and the method for subsequent solvent removing, have been proved to affect the performance of the resultant CSPs. Coating times and the concentration of coating solution, however, also makes a difference to CSPs' performance by changing the arrangement of cellulose derivatives while remaining the coating amount constant, have much less been studied before, and thereby, were systematically investigated in this work. Results showed that CSPs with more coating times exhibited higher chiral recognition and column efficiency, suggesting that resolution was determined by column efficiency herein. Afterwards, we also investigated the effect of coating amount on the performance of CSPs, and it was shown that the ability of enantio‐recognition did not increase all the time as the coating amount; and four of seven racemates achieved best resolution when the coating amount reached to 18.37%. At the end, the reproducibility of CDMPC‐coated CSPs were further confirmed by two methods, ie, reprepared the CSP‐0.15‐3 and reevaluated the effect of coating times. Abstract :