Real-time monitoring of T-cell-secreted interferon-γ for the diagnosis of tuberculosis. Issue 4 (4th July 2018)

• Record Type: Journal Article
• Authors: Wu, Changlin; He, Jianan; Li, Boan; Xu, YunQing; Gu, Dayong; Liu, Houming; Zhao, Dan; Shao, Chaopeng
• Is Part Of: Biotechnology, biotechnological equipment. Volume 32:Issue 4(2018)
• Page Start: 993
• Page End: 998
• Publication Date: 2018-07-04
• Subjects: Surface plasmon resonance -- protein microarray -- tubercle bacillus -- CD4+ T-cells -- IFN-γ -- label-free; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology -- instrumentation -- Periodicals; Periodicals; 660.605
• DOI: 10.1080/13102818.2018.1432416
• Languages: English
• ISSNs: 1310-2818

Abstract

ABSTRACT: In this paper, we report the development of a label-free biosensor, based on surface plasmon resonance, for real-time monitoring of captured human CD4 + T-cells, and their dynamic cytokine production upon specific antigen stimulation. Microarrays of CD4 + T-cells, and interferon gamma (IFN-γ) specific antibody (Ab) spots were printed side by side onto the poly(OEGMA- co -HEMA) matrix. The placement of CD4 + T-cells near the anti IFN-γ Ab-coated spots ensured a high local concentration of secreted IFN-γ for detection. We have demonstrated that this approach enables the detection of tuberculosis (TB) infection in clinical samples, with an overall sensitivity of 85.5% and an overall specificity of 97.7%.