Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier. Issue 1 (2nd January 2019)

• Record Type: Journal Article
• Authors: Zai, Khadijah; Ishihara, Narumi; Oguchi, Hiroyuki; Hirota, Masato; Kishimura, Akihiro; Mori, Takeshi; Hase, Koji; Katayama, Yoshiki
• Is Part Of: Journal of biomaterials science. Volume 30:Issue 1(2019)
• Page Start: 1
• Page End: 11
• Publication Date: 2019-01-02
• Subjects: Retinoic acid -- macrophages -- regulatory T cells -- inflammatory response -- nanostructured lipid carrier; Polymers -- Biocompatibility -- Periodicals; Biomedical materials -- Periodicals; 572.33
• DOI: 10.1080/09205063.2018.1493671
• Languages: English
• ISSNs: 0920-5063

Abstract

Abstract: Immunomodulatory function of all-trans retinoic acid (ATRA) has been gathering much attention for the therapy of autoimmune diseases. ATRA is a chemically unstable molecule which requires proper formulation for targeted delivery. Here we examined nanostructured lipid carrier (NLC) for the formulation of ATRA. NLC is a representative nanoparticle formulation especially suited for oral delivery. We established the preparation procedures of ATRA-containing NLC (NLC-RA) which minimizes the degradation of ATRA during the preparation process. NLC-RA thus obtained was taken up by macrophages and induced anti-inflammatory response via suppressing NF-κB signaling as well as via enhancing the production of anti-inflammatory cytokines. Moreover, NLC-RA enhanced differentiation of naïve T cells to regulatory T cells in the co-culture system with dendritic cells. These results suggest that NLC-RA is a promising alternative therapy for the autoimmune diseases especially intestinal bowel disease.