Three-dimensional origami paper-based device for portable immunoassay applications. Issue 4 (21st January 2019)

• Record Type: Journal Article
• Authors: Chen, Chung-An; Yeh, Wen-Shin; Tsai, Tsung-Ting; Li, Yu-De; Chen, Chien-Fu
• Is Part Of: Lab on a chip. Volume 19:Issue 4(2019)
• Page Start: 598
• Page End: 607
• Publication Date: 2019-01-21
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c8lc01255e
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : A three-dimensional surface-modified origami-paper-based analytical device for Staphylococcus aureus detection in highly viscous human synovial fluid. Abstract : In this study, we demonstrate a three-dimensional surface-modified origami-paper-based analytical device (3D-soPAD) for immunoassay applications. The platform enables the sequential steps of immunoassays to be easily performed using a folded, sliding paper design featuring multiple pre-stored reagents, allowing us to take advantage of the vertical diffusion of the analyte through the different paper layers. The cellulose substrate is composed of carboxymethyl cellulose modified with N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride and N -hydroxysuccinimide, which provide covalent bonding sites for bio-recognition molecules. After the optimization of the operation parameters, we determined the detection limit of the 3D-soPAD for human immunoglobulin G (HIgG) which can be as low as 0.01 ng mL −1, with a total turnaround time of 7 min. In order to study the long-term storage of the platform, anti-HIgG horseradish peroxidase (aHIgG-HRP) conjugates were stored by freeze-drying in sugar matrices composed of 10% sucrose/10% trehalose (w/w%) on the paper device, retaining 80% of their activity after 75 days of storage at 4 °C. To evaluate the performance of the paper device using real samples, we demonstrated the detection of protein A (a biomarker for Staphylococcus aureus infection) in highly viscous human synovial fluid. These results show that the proposed 3D-soPAD platform can provide sensitive, high-throughput, and on-site prognosis of infection in resource-limited settings.