Isolation and characterization of a bacteriophage and its potential to disrupt multi-drug resistant Pseudomonas aeruginosa biofilms. (March 2019)

• Record Type: Journal Article
• Authors: Yuan, Yuyu; Qu, Kunli; Tan, Demeng; Li, Xiaoyu; Wang, Lili; Cong, Cong; Xiu, Zhilong; Xu, Yongping
• Is Part Of: Microbial pathogenesis. Volume 128(2019)
• Page Start: 329
• Page End: 336
• Publication Date: 2019-03
• Subjects: Pseudomonas aeruginosa -- Bacteriophage -- Biofilm; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2019.01.032
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: A lytic Pseudomonas aeruginosa bacteriophage, vB_PaeM_LS1, was isolated and characterized herein. To examine the eligibility of bacteriophage vB_PaeM_LS1 as a therapeutic bacteriophage, we analysed its genome and compared it to similar bacteriophages. Genome of bacteriophage vB_PaeM_LS1 consisted of a linear, double-stranded DNA molecule 66, 095 bp in length and with 55.7% G + C content. Neighbor-joining analysis of the large subunit terminase showed that bacteriophage vB_PaeM_LS1 had similarity to the Pbunavirus genus. The potential of the lytic bacteriophage to disrupt Pseudomonas aeruginosa biofilms was assessed by scanning electron microscopy and bacterial counts. This study revealed that the bacteriophage vB_PaeM_LS1 with its lytic effect showed a high potential impact on the inhibition of the growth of Pseudomonas aeruginosa biofilm formation. Highlights: We present the characterization and genomic analysis of the lytic bacteriophage vB_PaeM_LS1. Several methods were used to assess the biofilm disruption potential of the bacteriophage. Development of bacteriophage resistance by Pseudomonas aeruginosa biofilm cells was determined.